Dr. SUBHANKAR SINGHA

Dr. Subhankar Singha, PhD

Assistant Professor

Centre for Health Science and Technology

Centre for Interdisciplinary Sciences

email: subhankar@jisiasr.org

Webpage: https://scholar.google.com/citations?user=JCu8I4AAAAAJ&hl=en

https://publons.com/researcher/1369721/subhankar-singha/

Ph.D., Dept. of Chemistry, Pohang University of Science and Technology (POSTECH), South Korea

M.Sc., Dept. of Chemistry, Indian Institute of Technology Kharagpur (IIT KGP), India

B.Sc., Dept. of Chemistry, Midnapore College, Vidyasagar University, India

Professional Experiences:
2019 – Present: Assistant Professor, JIS Institute of Advanced Studies and Research (JISIASR) Kolkata,
India

2015 – 2019: Research Assistant Professor, Dept. of Chemistry and School of Molecular Science
(BK21PLUS), POSTECH, South Korea

2013 – 2015: Post-Doctoral Research Assistant, Dept. of Chemistry and Center for Electro-Photo
Behaviors in Advanced Molecular Systems, POSTECH, South Korea

2008 – 2009: Research Chemist, Technology Improvement Group, Research & Development Centre,
Ranbaxy Laboratories Limited, Gurgaon, India

Research Interests:
Biosensors and chemosensors (fluorescence probes/assay kits development)

Two-photon fluorescent materials and two-photon fluorescence bioimaging

Smart materials for drug delivery and theranostics (disease diagnosis and therapy)

Probing biological signal transductions and neurodegenerative diseases

Investigation of redox biology of aging

Super-resolution imaging of proteins

Imaging guided surgery

Journal Publications (International):
1) S. Singha, Y. W. Jun, S. Sarkar, K. H. Ahn, An Endeavor in the Reaction-Based Approach to Fluorescent
Probes for Biorelevant Analytes: Challenges and Achievements, Accounts of Chemical Research, 2019, 52, 9, 2571-2581. (I. F. = 21.661)

2) K. H. Kim, S. Singha* (*corresponding author), Y. W. Jun, Y. J. Reo, H. R. Kim, H. G. Ryu, S. Bhunia, K. H.Ahn*, Far-red/near-infrared emitting, two-photon absorbing, and bio-stable amino-Si-pyronin dyes,
Chemical Science, 2019, 10, 9028-9037. (I.F. = 9.556)

3) S. W. Cho, A. S. Rao, S. Bhunia, Y. J. Reo, S. Singha* (*corresponding author), K. H. Ahn*, Ratiometric
fluorescence detection of Cu(II) with a keto-dipicolylamine ligand: a mechanistic implication, Sensors
And Actuators B-Chemical, 2019, 279, 204-212. (I.F. = 6.393)

4) P. Singh, D. Mukharjee, S. Singha, R. Das, S. K. Pal, Modulation of kinetic pathways of enzymesubstrate
interaction in a microfluidic channel: Nanoscopic water dynamics as a switch, Chemistry – A
European Journal, 2019, 25, 9728-9736. (I.F. = 5.160)

5) P. Singh, V. K. Sharma, S. Singha, V. G. Sakai, R. Mukhopadhyay, R. Das, S. K. Pal, Unraveling the role of monoolein in fluidity and dynamical response of a mixed cationic lipid bilayer, Langmuir, 2019, 35,4682–4692. (I.F. = 3.683)

6) T. Umme, S. Singha* (*corresponding author), H. Kim, Y. J. Reo, Y. W. Jun, A. Das, K. H. Ahn*, A
benzocoumarin based two-photon fluorescent probe for ratiometric detection of bisulfite, Sensors And
Actuators B-Chemical, 2018, 277, 576-583. (I.F. = 6.393)

7) H. G. Ryu, S. Singha* (*corresponding author), Y. W. Jun, Y. J. Reo, and K. H. Ahn*, Two-photon
fluorescent probe for hydrogen sulfide based on a red-emitting benzocoumarin dye, Tetrahedron
Letters, 2018, 59, 49-53. (I.F. = 2.259)

8) S. Singha* (*corresponding author), D. Kim, S. Bhuniya, T. Kumeria, Fluorescence analysis: from
sensing to imaging, Journal of Analytical Methods in Chemistry, 2018, 2654127. (I.F. = 1.589)

9) S. Sarkar, M. Santra, S. Singha, Y. W. Jun, Y. J. Reo, H. R. Kim, K. H. Ahn, Two-photon absorbing 8-
hydroxy-benzo[g]coumarins with giant Stokes shift: an environment-insensitive dye platform for probing
biomolecules, Journal of Materials Chemistry B, 2018, 6, 4446-4452. (I.F. = 5.047)

10) Y. W. Jun, S. Sarkar, S. Singha* (*corresponding author), Y. J. Reo, H. Kim, J.-J. Kim, Y.-T. Chang*, K. H.Ahn*, A two-photon fluorescent probe for ratiometric imaging of endogenous hypochlorous acid in live
cells and tissues, Chemical Communications, 2017, 53, 10800-10803. (I.F. = 6.164)

11) S. Singha, Y. W. Jun, J. Bae, K. H. Ahn, Ratiometric imaging of tissue by two-photon microscopy:
observation of a high level of formaldehyde around mouse intestinal crypts, Analytical Chemistry, 2017,
89, 3724-3731. (I.F. = 6.350)

12) P. Singh, S. Choudhury, S. Singha, Y. Jun, S. Chakraborty, J. Sengupta, R. Das, K. H. Ahn, S. K. Pal, A sensitive fluorescent probe for the polar solvation dynamics at protein–surfactant interfaces, Physical
Chemistry Chemical Physics, 2017, 19, 12237-12245. (I.F. = 3.567)

13) H. Seo, S. Singha, K. H. Ahn, Ratiometric fluorescence detection of anthrax biomarker with Eu(III)-
EDTA functionalized mixed poly(diacetylene) liposomes, Asian Journal of Organic Chemistry, 2017, 6,
1257-1263. (I.F. = 2.496)

14) C. Hine, H.-J. Kim, Y. Zhu, E. Harputlugil, A. Longchamp, M. S. Matos, P. Ramadoss, L. Brace, J. M.
Asara, C. K. Ozaki, S.-y. Cheng, S. Singha, K. H. Ahn, A. Kimmelman, F. M. Fisher, P. Pissios, D. J. Withers,C. Selman, R. Wang, K. Yen, V. Longo, P. Cohen, A. Bartke, J. J. Kopchick, R. Miller, A. Hollenberg, J. R.Mitchell, Hypothalamic-pituitary axis regulates hydrogen sulfide production, Cell Metabolism, 2017, 25,1320-1333. (I.F. = 22.415)

15) H.-J. Lee†, C.-W. Cho, H. Seo†, S. Singha† (†co-first author), Y. W. Jun, K.-H. Lee, Y. Jung, K.-T. Kim, S.Park, S. C. Bae, K. H. Ahn, A two-photon fluorescent probe for lysosomal zinc ions, Chemical
Communications, 2016, 52, 124-127. (I.F. = 6.164)

16) S. Singha, K. H. Ahn, Detection of ciprofloxacin in urine through sensitized lanthanide luminescence,
Sensors, 2016, 16, 2065. (I.F. = 3.031)

17) S. Singha, D. Kim, H. Seo, S. W. Cho, K. H. Ahn, Fluorescence sensing systems for gold and silver
species, Chemical Society Reviews, 2015, 44, 4367-4399. (I.F. = 40.443)

18) D. Kim†, H. Moon†, S. H. Baik†, S. Singha† (†co-first author), Y. W. Jun, T. Wang, K. H. Kim, B. S. Park,J. Jung, I. Mook-Jung, K. H. Ahn, Two-photon absorbing dyes with minimal autofluorescence in tissue
imaging: application to in vivo imaging of amyloid-beta plaques with a negligible background signal,
Journal of the American Chemical Society, 2015, 137, 6781-6789. (I.F. = 14.695)

19) S. Singha, D. Kim, B. Roy, S. Sambasivan, H. Moon, A. S. Rao, J. Y. Kim, T. Joo, J. W. Park, Y. M.
Rhee, T. Wang, K. H. Kim, Y. H. Shin, J. Jung, K. H. Ahn, A structural remedy toward bright dipolar
fluorophores in aqueous media, Chemical Science, 2015, 6, 4335-4342. (I.F. = 9.556)

20) S. Singha, D. Kim, H. Moon, T. Wang, K. H. Kim, Y. H. Shin, J. Jung, E. Seo, S.-J. Lee, K. H. Ahn, Toward a selective, sensitive, fast-responsive, and biocompatible two-photon probe for hydrogen sulfide in live cells, Analytical Chemistry, 2015, 87, 1188-1195. (I.F. = 6.350). • Highlighted in “Chemical & Engineering (C & EN) News” as “Sniffing out hydrogen sulfide in cells” on January 5, 2015. • Technology transferred & license agreement for commercialization with Merck Inc. (EMD Millipore), USA.

21) M. Tasior, D. Kim, S. Singha, M. Krzeszewski, K. H. Ahn, D. T. Gryko, π-Expanded coumarins:
synthesis, optical properties and applications, Journal of Materials Chemistry C, 2015, 3, 1421-1446. (I.F. = 6.641)

22) S. Singha, D. Kim, S. Rao, T. Wang, K. H. Kim, K. Lee, K. Kim, K. H. Ahn, Two-photon probes based on
arylsulfonyl azide: fluorescence detection and imaging of biothiols, Dyes and Pigments, 2013, 99, 308-
315. (I.F. = 4.018)

23) D. Kim, S. Singha, T. Wang, E. Seo, J. H. Lee, S. J. Lee, K. H. Kim, K. H. Ahn, In vivo two-photon
fluorescence imaging of fluoride with a desilylation-based reactive probe, Chemical Communications,
2012, 48, 10243-10245. (I.F. = 6.164)

24) A. S. Rao, S. Singha, W. Choi, K. H. Ahn, Studies on acedan-based mononuclear zinc complexes
toward selective fluorescent probes for pyrophosphate, Organic & Biomolecular Chemistry, 2012, 10,
8410-8417. (I.F. = 3.490)

25) L. Rajput, S. Singha, K. Biradha, Comparative structural studies on homologues of amides and reverse
amides: unprecedented 4-fold interpenetrated quartz network, new beta-sheet, and two-dimensional
layers, Crystal Growth & Design, 2007, 7, 2788-2795. (I.F. = 4.153)”

Conference Paper:
S. Singha, Y. W. Jun, J. Bae, Y. J. Reo, K. H. Ahn, Ratiometric tissue imaging for formaldehyde detection inmouse intestinal crypts, in Proceedings of the 3rd International Conference on Sensors Engineering and
Electronics Instrumentation Advances (SEIA’ 2017), 20-22 September, 2017, Moscow, Russia, pp.220-
222.

Book Chapter:
S. Singha* (*corresponding author), ‘Fluorescence detection of ciprofloxacin’ in M. S. Attia (ed.),
Ciprofloxacin: Biosynthesis, Applications, and Adverse Effects, Hauppage, NY: Nova Science Publishers,
2019, pp.265-293.

Patents:
“1) K. H. Ahn, D. Kim, S. Singha, One-photon and/or two-photon fluorescent probes for hydrogen sulfide,
biological imaging method of hydrogen sulfide using the same and synthesis method of the same, Organization: United States Patents and Trademark Office, Patent Application No: US 15/037,168
(Application date: 17.05.2016).

2) K. H. Ahn, D. Kim, H. Moon, S. Singha, B. Roy, S. Sunderraman, Novel two-photon absorbed
fluorophore and cellular imaging method using the same, Organization: United States Patents and
Trademark Office, Patent Application No: US 15/512,624 (Application date: 20.03.2017).

3) K. H. Ahn, Y. W. Jun, H. Kim, S. Singha, Y. J. Reo, Benzocoumarin based two-photon absorbing
fluorescent dyes, Organization: United States Patents and Trademark Office, Patent Application No: US
15/955,877 (Application date: 18.04.2018).

4) K. H. Ahn, D. Kim, S. Singha, One-photon and/or two-photon fluorescent probes for hydrogen sulfide,
biological imaging method of hydrogen sulfide using the same and synthesis method of the same,
Awarding Organization: Korean Intellectual Property Office, Registration number: KOR 10-1481921
(Registration date: 06.01.2015).  Technology transferred & license agreement for commercialization
with Merck Inc., USA.

5) K. H. Ahn, D. Kim, H. Moon, S. Singha, B. Roy, S. Sunderraman, Novel two-photon absorbed
fluorophore and cellular imaging method using the same, Awarding Organization: Korean Intellectual
Property Office, Registration number: KOR 10-1662444 (Registration date: 27.09.2016).

6) K. H. Ahn, Y. W. Jun, J. Bae, H. G. Ryu, S. Singha, Probes for detecting formaldehyde or measuring
formaldehyde concentration, two-photon ratiometric fluorescence imaging and concentration
measurement of formaldehyde in cell or tissue using the same, Organization: Korean Intellectual
Property Office, Patent Application No: KOR 10-2016-0124841 (Application date: 28.09.2016).

7) K. H. Ahn, Y. W. Jun, H. Kim, S. Singha, Y. J. Reo, Benzocoumarin based two-photon absorbing
fluorescent dyes, Organization: Korean Intellectual Property Office, Patent Application No: KOR 10-2017-
0078999 (Application date: 22.06.2017).

8) K. H. Ahn, H. G. Ryu, Y. W. Jun, K. H. Kim, S. Singha, One- or two-photon absorbing fluorescent dyes
based on amino-Si-pyronin compound and the uses thereof; Organization: Korean Intellectual Property
Office, Patent Application No: KOR 10-2018-0102776 (Application date: 30.08.2018).

Sponsored Project Undertaken:

Project Title: Hydrogen Sulfide Triggered Drug Delivery for Theranostics

Principal Investigator (PI): Subhankar Singha

Sponsored by: National Research Foundation (NRF) of Korea for Basic Science Research ProgramAward
no: 2018R1D1A1B07051403; Periods: Jun, 2018 ~ May, 2023 (5 years)

Total grant amount: 250,000 Thousand Korean Won (approx. 220,000 USD)

Technology Transfer & License Agreement
(For Product Commercialization) :

Industry: EMD Millipore, Merck (Catalogue Number: 534329), USA

Product name: H₂S Fluorescent Probe, P3 – Calbiochem®

Description: A cell-permeable, specific, sensitive, fast-responsive fluorescent turn-on H₂S probe.
Monitors endogenous [H₂S] in live cells by two-photon imaging

Reference: S. Singha, et al. Analytical Chemistry, 2015, 87, 1188

Conference Presentations (Oral):

Invited Lecture:

“Fluorescence Bioimaging and Biosensing: Future Perspectives in Theranostics”, Invited lecture, National
Institute of Pharmaceutical Education and Research (NIPER) – Kolkata, 31st Aug, 2018.

Honors, Awards, & Achievements:

Awarded Fund from National Research Foundation (NRF), South Korea for Basic Science Research
Program for 5 years, Project title: Hydrogen sulfide triggered drug delivery for theranostics, Award No.:
2018R1D1A1B07051403 (Jun, 2018-May, 2023).

Research Assistant Professor Fellowship (financial support) from Global Research Laboratory (GRL)
program, South Korea (Oct, 2015-present).

Post-Doctoral Research fellowship from Center for Electro-Photo Behaviors in Advanced Molecular
Systems (EPB center), South Korea (Aug, 2013-Feb, 2015).

CSIR-UGC NET (National Eligibility Test) Examination qualified (Exam date: 24th Dec, 2006) in India.
MCM (Merit cum Means) Scholarship from IIT Kharagpur, 2005-2007.

All India Rank 59th in JAM (Joint Admission Test for M.Sc. in IITs), 2005.

Editorial Experiences:

“Lead Guest Editor for Journal of Analytical Methods in Chemistry (Hindawi Publishing Corporation) for
the special publication issue on “Fluorescence analysis: From sensing to imaging” (2017-2018).

Academic Editor for Journal of Analytical Methods in Chemistry (2017-2018).”

Reviewer Experiences:

Reviewer for-

Royal Society of Chemistry (Journal of Materials Chemistry B, Organic & Biomolecular Chemistry,
Photochemical & Photobiological Science),

Elsevier (Sensors and Actuators B: Chemical, Journal of Luminescence, Microchemical Journal),

MDPI (Molecules, Chemosensors, Sensors, Applied Science, Diagnostics, Dentistry Journal),

Bentham Science (Current Analytical Chemistry),

Sciencedomain International (Journal of Pharmaceutical Research International)”