Dr. SUBHANKAR SINGHA

Assistant Professor

Centre for Interdisciplinary Sciences (CIS), JISIASR Kolkata

Email: subhankar@jisiasr.org

Google Scholar: https://scholar.google.com/citations?user=JCu8I4AAAAAJ&hl=en;

Publons: https://publons.com/researcher/1369721/subhankar-singha/

 Ph.D., Dept. of Chemistry, Pohang University of Science and Technology (POSTECH), South Korea

M.Sc., Dept. of Chemistry, Indian Institute of Technology Kharagpur (IIT KGP), India

B.Sc., Dept. of Chemistry, Midnapore College, Vidyasagar University, India

Professional Experiences:

2019 – Present: Assistant Professor, JIS Institute of Advanced Studies and Research (JISIASR) Kolkata,
India

2015 – 2019: Research Assistant Professor, Dept. of Chemistry and School of Molecular Science
(BK21PLUS), POSTECH, South Korea

2013 – 2015: Post-Doctoral Research Assistant, Dept. of Chemistry and Center for Electro-Photo
Behaviors in Advanced Molecular Systems, POSTECH, South Korea

2008 – 2009: Research Chemist, Technology Improvement Group, Research & Development Centre,
Ranbaxy Laboratories Limited, Gurgaon, India

Research Interests:

Bioorganic Chemistry; Biosensors and chemosensors (fluorescence probes/assay kits development); Two-photon fluorescent materials and two-photon fluorescence bioimaging; Smart materials for drug delivery and theranostics (disease diagnosis and therapy); Probing biological signal transductions and neurodegenerative diseases; Investigation of redox biology of aging; Super-resolution imaging of proteins; Imaging guided surgery

Journal Publications (International):

  • Sarkar, H. Lee, H. G. Ryu, S. Singha, Y. M. Lee, Y. J. Reo, Y. W. Jun, K. H. Kim, W. J. Kim, K. H. Ahn, A Study on Hypoxia Susceptibility of Organ Tissues by Fluorescence Imaging with a Ratiometric, ACS Sensors, 2021, 6, 148–155 (I.F. = 7.711).
  • Mukherjee, P. Singh, S. Singh, D. Singh Roy, S. Singha, U. Pal, J. Sengupta, R. J. Obaid, S. A. Ahmed, T. Saha Dasgupta, R. Das, S. K. Pal, Host Assisted Molecular Recognition by Human Serum Albumin: Study of Molecular Recognition Controlled Protein/Drug Mimic Binding in a Microfluidic Channel, International Journal of Biological Macromolecules, 2021, 176, 137-144 (I.F. = 6.953).
  • J. Reo, Y. W. Jun, S. W. Cho, J. Jeon, H. Roh, S. Singha, M. Dai, S. Sarkar, H. R. Kim, S. Kim, Y. Jin, Y. L. Jung, Y. J. Yang, C. Ban, J. Joo, K. H. Ahn, Systematic study on discrepancy of fluorescent properties between in solutions and in cells: Super-bright, environment-insensitive benzocoumarin dyes, Chemical Communication, 2020, 56, 10556-10559 (I.F. = 6.222).
  • N. Carter, M. T. G. Gibbins, M. E. Barrios-Llerena, S. Wilkie, P. Freddolino, M. Libiad, V. Vitvitsky, B. Emerson, T. LeBihan, M. Brice, H. Su, S. Denham, N. Z.M. Homer, C. Mc Fadden, A. Tailleux, N. Faresse, T. Sulpice, F. Briand, T. Gillingwater, K. H. Ahn, S. Singha, C. McMaster, R. C. Hartley, B. Staels, G. Gray, A. J. Finch, C. Selman, R. Banerjee, N. M. Morton, The hepatic compensatory response to elevated systemic sulfide impairs medium chain fat oxidation and promotes diabetes, bioRxiv, 2020, doi: 10.1101/2020.04.27.064287.
  • Singha, Y. W. Jun, S. Sarkar, K. H. Ahn, An Endeavor in the Reaction-Based Approach to Fluorescent
    Probes for Biorelevant Analytes: Challenges and Achievements, Accounts of Chemical Research, 2019,
    52, 2571-2581 (I. F. = 22.384).
  • H. Kim, S. Singha* (*corresponding author), Y. W. Jun, Y. J. Reo, H. R. Kim, H. G. Ryu, S. Bhunia, K. H.Ahn*, Far-red/near-infrared emitting, two-photon absorbing, and bio-stable amino-Si-pyronin dyes,
    Chemical Science, 2019, 10, 9028-9037 (I.F. = 9.825).
  • Singh, D. Mukherjee, S. Singha, V. K. Sharma, I. Althagafi, S A. Ahmed, R. Mukhopadhyay, R. Das, S. K. Pal, Probing relaxation dynamics of a cationic lipid based non-viral carrier: a time-resolved fluorescence study, RSC Advances, 2019, 9, 35549-35558 (I.F. = 3.361).
  • W. Cho, A. S. Rao, S. Bhunia, Y. J. Reo, S. Singha* (*corresponding author), K. H. Ahn*, Ratiometric
    fluorescence detection of Cu(II) with a keto-dipicolylamine ligand: a mechanistic implication, Sensors and Actuators B-Chemical, 2019, 279, 204-212 (I.F. = 7.460).
  • Singh, D. Mukharjee, S. Singha, R. Das, S. K. Pal, Modulation of kinetic pathways of enzymesubstrate
    interaction in a microfluidic channel: Nanoscopic water dynamics as a switch, Chemistry – A
    European Journal
    , 2019, 25, 9728-9736 (I.F. = 5.236).
  • Singh, V. K. Sharma, S. Singha, V. G. Sakai, R. Mukhopadhyay, R. Das, S. K. Pal, Unraveling the role of monoolein in fluidity and dynamical response of a mixed cationic lipid bilayer, Langmuir, 2019, 35,4682–4692 (I.F. = 3.882).
  • Umme, S. Singha* (*corresponding author), H. Kim, Y. J. Reo, Y. W. Jun, A. Das, K. H. Ahn*, A
    benzocoumarin based two-photon fluorescent probe for ratiometric detection of bisulfite, Sensors and
    Actuators B-Chemical
    , 2018, 277, 576-583 (I.F. = 7.460).
  • G. Ryu, S. Singha* (*corresponding author), Y. W. Jun, Y. J. Reo, and K. H. Ahn*, Two-photon
    fluorescent probe for hydrogen sulfide based on a red-emitting benzocoumarin dye, Tetrahedron
    Letters
    , 2018, 59, 49-53 (I.F. = 2.415).
  • Singha* (*corresponding author), D. Kim, S. Bhuniya, T. Kumeria, Fluorescence analysis: from
    sensing to imaging, Journal of Analytical Methods in Chemistry, 2018, 2654127 (I.F. = 2.193).
  • Sarkar, M. Santra, S. Singha, Y. W. Jun, Y. J. Reo, H. R. Kim, K. H. Ahn, Two-photon absorbing 8-
    hydroxy-benzo[g]coumarins with giant Stokes shift: an environment-insensitive dye platform for probing
    biomolecules, Journal of Materials Chemistry B, 2018, 6, 4446-4452 (I.F. = 6.331).
  • W. Jun, S. Sarkar, S. Singha* (*corresponding author), Y. J. Reo, H. Kim, J.-J. Kim, Y.-T. Chang*, K. H.Ahn*, A two-photon fluorescent probe for ratiometric imaging of endogenous hypochlorous acid in live
    cells and tissues, Chemical Communications, 2017, 53, 10800-10803 (I.F. = 6.222).
  • Singha, Y. W. Jun, J. Bae, K. H. Ahn, Ratiometric imaging of tissue by two-photon microscopy:
    observation of a high level of formaldehyde around mouse intestinal crypts, Analytical Chemistry, 2017,
    89, 3724-3731 (I.F. = 6.986).
  • Singh, S. Choudhury, S. Singha, Y. Jun, S. Chakraborty, J. Sengupta, R. Das, K. H. Ahn, S. K. Pal, A sensitive fluorescent probe for the polar solvation dynamics at protein–surfactant interfaces, Physical
    Chemistry Chemical Physics
    , 2017, 19, 12237-12245 (I.F. = 3.676).
  • Seo, S. Singha, K. H. Ahn, Ratiometric fluorescence detection of anthrax biomarker with Eu(III)-
    EDTA functionalized mixed poly(diacetylene) liposomes, Asian Journal of Organic Chemistry, 2017, 6,
    1257-1263 (I.F. = 3.319).
  • Hine, H.-J. Kim, Y. Zhu, E. Harputlugil, A. Longchamp, M. S. Matos, P. Ramadoss, L. Brace, J. M.
    Asara, C. K. Ozaki, S.-y. Cheng, S. Singha, K. H. Ahn, A. Kimmelman, F. M. Fisher, P. Pissios, D. J. Withers,C. Selman, R. Wang, K. Yen, V. Longo, P. Cohen, A. Bartke, J. J. Kopchick, R. Miller, A. Hollenberg, J. R.Mitchell, Hypothalamic-pituitary axis regulates hydrogen sulfide production, Cell Metabolism, 2017, 25,1320-1333 (I.F. = 27.287).
  • -J. Lee†, C.-W. Cho, H. Seo†, S. Singha† (†co-first author), Y. W. Jun, K.-H. Lee, Y. Jung, K.-T. Kim, S.Park, S. C. Bae, K. H. Ahn, A two-photon fluorescent probe for lysosomal zinc ions, Chemical Communications, 2016, 52, 124-127 (I.F. = 6.222).
  • Singha, K. H. Ahn, Detection of ciprofloxacin in urine through sensitized lanthanide luminescence,
    Sensors, 2016, 16, 2065 (I.F. = 3.576).
  • Singha, D. Kim, H. Seo, S. W. Cho, K. H. Ahn, Fluorescence sensing systems for gold and silver
    species, Chemical Society Reviews, 2015, 44, 4367-4399 (I.F. = 54.564).
  • Kim†, H. Moon†, S. H. Baik†, S. Singha† (†co-first author), Y. W. Jun, T. Wang, K. H. Kim, B. S. Park,J. Jung, I. Mook-Jung, K. H. Ahn, Two-photon absorbing dyes with minimal autofluorescence in tissue
    imaging: application to in vivo imaging of amyloid-beta plaques with a negligible background signal,
    Journal of the American Chemical Society, 2015, 137, 6781-6789 (I.F. = 15.419).
  • Singha, D. Kim, B. Roy, S. Sambasivan, H. Moon, A. S. Rao, J. Y. Kim, T. Joo, J. W. Park, Y. M.
    Rhee, T. Wang, K. H. Kim, Y. H. Shin, J. Jung, K. H. Ahn, A structural remedy toward bright dipolar
    fluorophores in aqueous media, Chemical Science, 2015, 6, 4335-4342 (I.F. = 9.825).
  • Singha, D. Kim, H. Moon, T. Wang, K. H. Kim, Y. H. Shin, J. Jung, E. Seo, S.-J. Lee, K. H. Ahn, Toward a selective, sensitive, fast-responsive, and biocompatible two-photon probe for hydrogen sulfide in live cells, Analytical Chemistry, 2015, 87, 1188-1195 (I.F. = 6.986). #Highlighted in “Chemical & Engineering (C & EN) News” as “Sniffing out hydrogen sulfide in cells” on January 5, 2015. #Technology transferred & license agreement for commercialization with Merck Inc. (EMD Millipore), USA.
  • Tasior, D. Kim, S. Singha, M. Krzeszewski, K. H. Ahn, D. T. Gryko, π-Expanded coumarins:
    synthesis, optical properties and applications, Journal of Materials Chemistry C, 2015, 3, 1421-1446 (I.F. = 7.393).
  • Singha, D. Kim, S. Rao, T. Wang, K. H. Kim, K. Lee, K. Kim, K. H. Ahn, Two-photon probes based on
    arylsulfonyl azide: fluorescence detection and imaging of biothiols, Dyes and Pigments, 2013, 99, 308-
    315 (I.F. = 4.889).
  • Kim, S. Singha, T. Wang, E. Seo, J. H. Lee, S. J. Lee, K. H. Kim, K. H. Ahn, In vivo two-photon
    fluorescence imaging of fluoride with a desilylation-based reactive probe, Chemical Communications,
    2012, 48, 10243-10245 (I.F. = 6.222).
  • S. Rao, S. Singha, W. Choi, K. H. Ahn, Studies on acedan-based mononuclear zinc complexes
    toward selective fluorescent probes for pyrophosphate, Organic & Biomolecular Chemistry, 2012, 10,
    8410-8417 (I.F. = 3.876).
  • Rajput, S. Singha, K. Biradha, Comparative structural studies on homologues of amides and reverse
    amides: unprecedented 4-fold interpenetrated quartz network, new beta-sheet, and two-dimensional
    layers, Crystal Growth & Design, 2007, 7, 2788-2795 (I.F. = 4.076).

Conference Paper:

  • Singha, Y. W. Jun, J. Bae, Y. J. Reo, K. H. Ahn, Ratiometric tissue imaging for formaldehyde detection inmouse intestinal crypts, in Proceedings of the 3rd International Conference on Sensors Engineering and
    Electronics Instrumentation Advances (SEIA’ 2017), 20-22 September, 2017, Moscow, Russia, pp.220-
    222.

 Book Chapter:

  • Singha* (*corresponding author), ‘Fluorescence detection of ciprofloxacin’ in M. S. Attia (ed.),
    Ciprofloxacin: Biosynthesis, Applications, and Adverse Effects, Hauppage, NY: Nova Science Publishers,
    2019, pp.265-293.

 Patents:

  • H. Ahn, D. Kim, S. Singha, One-photon and/or two-photon fluorescent probes for hydrogen sulfide, biological imaging method of hydrogen sulfide using the same and synthesis method of the same, United States Patent Application Publication, Patent Application No: US 15/037,168 (Application date: 17.05.2016).
  • H. Ahn, D. Kim, H. Moon, S. Singha, B. Roy, S. Sunderraman, Novel two-photon absorbed fluorophore and cellular imaging method using the same, United States Patent Application Publication, Patent Application No: US 15/512,624 (Application date: 20.03.2017).
  • H. Ahn, Y. W. Jun, H. Kim, S. Singha, Y. J. Reo, Benzocoumarin based two-photon absorbing fluorescent dyes, United States Patent Application Publication, Patent Application No: US 15/955,877 (Application date: 18.04.2018).
  • H. Ahn, H. G. Ryu, Y. W. Jun, K. H. Kim, S. Singha, Amino-Si-pyronin compound-based one- or two-photon absorption fluorescent substance and use thereof, United States Patent Application Publication, Patent Application No: US 16/642,439 (Publication Date: Aug 6, 2020).
  • H. Ahn, D. Kim, S. Singha, One-photon and/or two-photon fluorescent probes for hydrogen sulfide,
    biological imaging method of hydrogen sulfide using the same and synthesis method of the same,
    Awarding Organization: Korean Intellectual Property Office, Registration number: KOR 10-1481921
    (Registration date: 06.01.2015). # Technology transferred & license agreement for commercialization
    with Merck Inc., USA.
  • H. Ahn, D. Kim, H. Moon, S. Singha, B. Roy, S. Sunderraman, Novel two-photon absorbed
    fluorophore and cellular imaging method using the same, Awarding Organization: Korean Intellectual
    Property Office, Registration number: KOR 10-1662444 (Registration date: 27.09.2016).
  • H. Ahn, Y. W. Jun, J. Bae, H. G. Ryu, S. Singha, Probes for detecting formaldehyde or measuring
    formaldehyde concentration, two-photon ratiometric fluorescence imaging and concentration
    measurement of formaldehyde in cell or tissue using the same, Organization: Korean Intellectual
    Property Office, Patent Application No: KOR 10-2016-0124841 (Application date: 28.09.2016).
  • H. Ahn, Y. W. Jun, H. Kim, S. Singha, Y. J. Reo, Benzocoumarin based two-photon absorbing
    fluorescent dyes, Organization: Korean Intellectual Property Office, Patent Application No: KOR 10-2017-
    0078999 (Application date: 22.06.2017).
  • H. Ahn, H. G. Ryu, Y. W. Jun, K. H. Kim, S. Singha, One- or two-photon absorbing fluorescent dyes
    based on amino-Si-pyronin compound and the uses thereof; Organization: Korean Intellectual Property
    Office, Patent Application No: KOR 10-2018-0102776 (Application date: 30.08.2018).

Sponsored Project Undertaken:

Project Title: Hydrogen Sulfide Triggered Drug Delivery for Theranostics

Principal Investigator (PI): Dr. Subhankar Singha

Sponsored by: National Research Foundation (NRF) of Korea for Basic Science Research Program

Award No: 2018R1D1A1B07051403; Periods: Jun, 2018 ~ May, 2023 (5 years)

Total grant amount: 250,000, 000 Korean Won (approx. 220,000 USD)

Technology Transfer & License Agreement (For Product Commercialization):

Industry: EMD Millipore, Merck (Catalogue Number: 534329), USA

Product name: H₂S Fluorescent Probe, P3 – Calbiochem®

Description: A cell-permeable, specific, sensitive, fast-responsive fluorescent turn-on H₂S probe. Monitors endogenous [H₂S] in live cells by two-photon imaging.

Reference: S. Singha, et al. Analytical Chemistry, 2015, 87, 1188

Membership of Professional Bodies:

  • Community Member (Membership Number: 31340778) of the American Chemical Society (ACS), USA (2020-present).
  • Affiliate Member (Membership ID: 704682) of the Royal Society of Chemistry (RSC), London, UK (2021-present).

Honors, Awards, & Achievements:

  • Awarded Fund from National Research Foundation (NRF), South Korea for Basic Science Research
    Program for 5 years, Project title: Hydrogen sulfide triggered drug delivery for theranostics, Award No.:
    2018R1D1A1B07051403 (Jun, 2018-May, 2023).
  • Research Assistant Professor Fellowship (financial support) from Global Research Laboratory (GRL)
    program, South Korea (Oct, 2015-present).
  • Post-Doctoral Research fellowship from Center for Electro-Photo Behaviors in Advanced Molecular
    Systems (EPB center), South Korea (Aug, 2013-Feb, 2015).
  • CSIR-UGC NET (National Eligibility Test) Examination qualified (Exam date: 24th Dec, 2006) in India.
    MCM (Merit cum Means) Scholarship from IIT Kharagpur, 2005-2007.
  • All India Rank 59th in JAM (Joint Admission Test for M.Sc. in IITs), 2005.

Editorial Experiences:

  • Review Editor for Frontiers in Environmental Chemistry (Section: Environmental Analytical Methods) (2020-present).
  • Topic Editor for Polymers (MDPI) (2020-present).
  • Topic Editor for Liquids (MDPI) (2020-present).
  • Lead Guest Editor for Journal of Analytical Methods in Chemistry (Hindawi Publishing Corporation) for
    the special publication issue on “Fluorescence analysis: From sensing to imaging” (2017-2018).
  • Academic Editor for Journal of Analytical Methods in Chemistry (2017-2018).

Reviewer Experiences:

Reviewed journals of internationally reputed publishers, like –

  1. American Chemical Society (ACS)
  2. Royal Society of Chemistry (RCS)
  3. Elsevier
  4. Wiley
  5. Springer Nature
  6. MDPI
  7. Bentham Science
  8. Sciencedomain International