DR. SUBHANKAR SINGHA

Department: Centre for Health Science & Technology

JIS Institute of Advanced Studies & research

HC9M+5F2 ARC Water front(N/AC, Ring Rd, GP Block, Sector V, Bidhannagar, Kolkata, West Bengal 700091

Associate Professor & Head, Centre for Interdisciplinary Sciences, JISIASR
MSc (IIT Kharagpur),
PhD (POSTECH, S. Korea),
Post-Doc & Research Professorship (POSTECH, S. Korea)

Email: subhankar@jisiasr.org
Google Scholar: https://scholar.google.com/citations?user=JCu8I4AAAAAJ&hl=en;
Publons: https://publons.com/researcher/1369721/subhankar-singha/

Ph.D., Dept. of Chemistry, Pohang University of Science and Technology (POSTECH), South Korea
M.Sc., Dept. of Chemistry, Indian Institute of Technology Kharagpur (IIT KGP), India
B.Sc., Dept. of Chemistry, Midnapore College, Vidyasagar University, India

Professional Experiences:

2023 – Present: Associate Professor, JIS Institute of Advanced Studies and Research (JISIASR), JIS University, Kolkata, India
2019 – 2022: Assistant Professor, JIS Institute of Advanced Studies and Research (JISIASR), JIS University,Kolkata,India
2015–2019: Research Assistant Professor, Dept. of Chemistry and School of Molecular Science
(BK21PLUS), POSTECH, South Korea
2013–2015: Post-Doctoral Research Assistant, Dept. of Chemistry and Center for Electro-Photo
Behaviors in Advanced Molecular Systems, POSTECH, South Korea
2008–2009: Research Chemist, Technology Improvement Group, Research & Development Centre,
Ranbaxy Laboratories Limited, Gurgaon, India

Research Interests:

Bioorganic Chemistry: Biosensors and Chemosensors (fluorescence probes/assay kits development)
Two-photon fluorescent materials and two-photon fluorescence bioimaging
Smart materials for drug delivery and theranostics (disease diagnosis and therapy)

Journal Publications (International):
@ JISIASR, Kolkata

S. Sarkar, A. Shil, Y. W. Jun, Y. J. Yang, W. Choi, S. Singha*, K. H. Ahn*, Interactive Twin Intramolecular Hydrogen Bonds Enable Bright, S‐Blue Emissive, Environment‐Insensitive Single‐Benzene Fluorophores, Advanced Functional Materials, Just Published Online, Jul 2023, doi.org/10.1002/adfm.202304507.(Impact Factor = 19.0)
S. Sarkar, A. Shil, Y. Jung, S. Singha, K. H. Ahn, Rapid point-of-care quantification of human serum albumin in urine based on ratiometric fluorescence signaling driven by intramolecular H-bonding, ACS Sensors, 2022, 7, 3790−3799. (Impact Factor = 8.9)
U. Samanta, S. Singha*, Quantifications of Endogenous Formaldehyde Levels in Mouse Organ Tissues through Two-Photon Fluorescence Bioimaging, Journal of Science, 2022, 5 (2), 22.
S. Sarakar, A. Shil, M. Nandy, S. Singha, Y. J. Reo, Y. J. Yang, K. H. Ahn, Rationally Designed Two-Photon Ratiometric Elastase Probe for Investigating Inflammatory Bowel Disease, Analytical Chemistry, 2022, 94, 1373–1381.(Impact Factor = 7.4)
K. H. Kim, S. J. Kim, S. Singha, Y. J. Yang, S. K. Park, K. H. Ahn, Ratiometric detection of hypochlorous acid in brain tissues of neuroinflammation and maternal immune activation models with a deep-red/near-infrared emitting probe, ACS Sensors, 2021, 6, 9, 3253–3261.(Impact Factor = 8.9)
S. Sarkar, H. Lee, H. G. Ryu, S. Singha, Y. M. Lee, Y. J. Reo, Y. W. Jun, K. H. Kim, W. J. Kim, K. H. Ahn, A study on hypoxia susceptibility of organ tissues by fluorescence imaging with a ratiometricnitroreductase probe, ACS Sensors, 2021, 6, 148–155.(Impact Factor = 8.9)
D. Mukherjee, P. Singh, S. Singh, D. Singh Roy, S. Singha, U. Pal, J. Sengupta, R. J. Obaid, S. A. Ahmed, T. SahaDasgupta, Ranjan Das, S. K. Pal, Host assisted molecular recognition by human serum albumin: Study of molecular recognition-controlled protein/drug mimic binding in a microfluidic channel, International Journal of Biological Macromolecules, 2021, 176, 137-144.(Impact Factor = 8.2)
R. N. Carter, M. T. G. Gibbins, M. E. Barrios-Llerena, S. Wilkie, P. Freddolino, M. Libiad, V. Vitvitsky, B. Emerson, T. LeBihan, M. Brice, H. Su, S. Denham, N. Z.M. Homer, C. Mc Fadden, A. Tailleux, N. Faresse, T. Sulpice, F. Briand, T. Gillingwater, K. H. Ahn, S. Singha, C. McMaster, R. C. Hartley, B. Staels, G. Gray, A. J. Finch, C. Selman, R. Banerjee, N. M. Morton, The hepatic compensatory response to elevated systemic sulfide impairs medium chain fat oxidation and promotes diabetes, Cell Reports, 2021, 37,109958. (Impact Factor = 8.8)
Y. J. Reo, Y. W. Jun, S. W. Cho, J. Jeon, H. Roh, S. Singha, M. Dai, S. Sarkar, H. R. Kim, S. Kim, Y. Jin, Y. L. Jung, Y. J. Yang, C. Ban, J. Joo, K. H. Ahn, A systematic study on discrepancy of fluorescent properties between in solutions and in cells: Super-bright, environment-insensitive benzocoumarin dyes, Chemical Communication, 2020, 56, 10556-10559. (Impact Factor = 4.9)
P. Singh, D. Mukherjee, S. Singha, V. K. Sharma, I. I. Althagafid, S. A. Ahmed, R. Mukhopadhyay, R. Das, S. K. Pal, Probing relaxation dynamics of a cationic lipid based non-viral carrier: a time-resolved fluorescence study, RSC Advances, 2019, 9, 35549-35558. (Impact Factor = 3.9)
S. Singha, Y. W. Jun, S. Sarkar, K. H. Ahn, An endeavor in the reaction-based approach to fluorescent probes for biorelevant analytes: Challenges and achievements, Accounts of Chemical Research, 2019, 52, 2571-2581. (Impact Factor = 18.3)

@ POSTECH, South Korea

K. H. Kim, S. Singha*, Y. W. Jun, Y. J. Reo, H. R. Kim, H. G. Ryu, S. Bhunia, K. H. Ahn*, Far-red/near-infrared emitting, two-photon absorbing, and bio-stable amino-Si-pyronin dyes, Chemical Science, 2019, 10, 9028-9037. (Impact Factor = 8.4)
S. W. Cho, A. S. Rao, S. Bhunia, Y. J. Reo, S. Singha*, K. H. Ahn*, Ratiometric fluorescence detection of Cu(II) with a keto-dipicolylamine ligand: A mechanistic implication, Sensors and Actuators B-Chemical, 2019, 279, 204-212.(Impact Factor = 8.4)
P. Singh, D. Mukharjee, S. Singha, R. Das, S. K. Pal, Modulation of kinetic pathways of enzyme-substrate interaction in a microfluidic channel: Nanoscopic water dynamics as a switch, Chemistry–A European Journal, 2019, 25, 9728-9736. (Impact Factor = 4.3)
P. Singh, V. K. Sharma, S. Singha, V. G. Sakai, R. Mukhopadhyay, R. Das, S. K. Pal, Unraveling the Role of Monoolein in Fluidity and Dynamical Response of a Mixed Cationic Lipid Bilayer, Langmuir, 2019, 35, 4682–4692.(Impact Factor = 3.9)
T. Umme, S. Singha*, H. Kim, Y. J. Reo, Y. W. Jun, A. Das, K. H. Ahn*, A benzocoumarin based two-photon fluorescent probe for ratiometric detection of bisulfite, Sensors and Actuators B-Chemical, 2018, 277, 576-583.(Impact Factor = 8.4)
H. G. Ryu, S. Singha*, Y. W. Jun, Y. J. Reo, and K. H. Ahn*, Two-photon fluorescent probe for hydrogen sulfide based on a red-emitting benzocoumarin dye, Tetrahedron Letters, 2018, 59, 49-53 (Impact Factor = 2.032).
S. Singha*, D. Kim, S. Bhuniya, T. Kumeria, Fluorescence Analysis: From Sensing to Imaging, Journal of Analytical Methods in Chemistry, 2018, 2654127.(Impact Factor = 2.6)
S. Sarkar, M. Santra, S. Singha, Y. W. Jun, Y. J. Reo, H. R. Kim, K. H. Ahn, Two-photon absorbing 8-hydroxy-benzo[g]coumarins with giant Stokes shift: An environment-insensitive dye platform for probing biomolecules, Journal of Materials Chemistry B, 2018, 6, 4446-4452.(Impact Factor = 7.0)
S. Singha, Y. W. Jun, J. Bae, K. H. Ahn, Ratiometric imaging of tissue by two-photon microscopy: Observation of a high level of formaldehyde around mouse intestinal crypts, Analytical Chemistry, 2017, 89, 3724-3731.(Impact Factor = 7.4)
Y. W. Jun, S. Sarkar, S. Singha*, Y. J. Reo, H. Kim, J.-J. Kim, Y.-T. Chang*, K. H. Ahn*, A two-photon fluorescent probe for ratiometricimaging of endogenous hypochlorous acid in live cells and tissues, Chemical Communications, 2017, 53, 10800-10803.(Impact Factor = 4.9)
P. Singh, S. Choudhury, S. Singha, Y. Jun, S. Chakraborty, J. Sengupta, R. Das, K. H. Ahn, S. K. Pal, A sensitive fluorescent probe for the polar solvation dynamics at protein–surfactant interfaces, Physical Chemistry Chemical Physics, 2017, 19, 12237-12245. (Impact Factor = 3.3)
H. Seo, S. Singha, K. H. Ahn,Ratiometric fluorescence detection of anthrax biomarker with Eu(III)-EDTA functionalized mixed poly(diacetylene) liposomes, Asian Journal of Organic Chemistry, 2017, 6, 1257-1263. (Impact Factor = 2.7)
C. Hine, H.-J. Kim, Y. Zhu, E. Harputlugil, A. Longchamp, M. S. Matos, P. Ramadoss, L. Brace, J. M. Asara, C. K. Ozaki, S.-y. Cheng, S. Singha, K. H. Ahn, A. Kimmelman, F. M. Fisher, P. Pissios, D. J. Withers, C. Selman, R. Wang, K. Yen, V. Longo, P. Cohen, A. Bartke, J. J. Kopchick, R. Miller, A. Hollenberg, J. R. Mitchell, Hypothalamic-pituitary axis regulates hydrogen sulfide production, Cell Metabolism, 2017, 25, 1320-1333.(Impact Factor = 29.0)
H.-J. Lee†, C.-W. Cho, H. Seo†, S. Singha† (†co-first author), Y. W. Jun, K.-H. Lee, Y. Jung, K.-T. Kim, S. Park, S. C. Bae, K. H. Ahn, A two-photon fluorescent probe for lysosomal zinc ions, Chemical Communications, 2016, 52, 124-127.(Impact Factor = 4.9)
S. Singha, K. H. Ahn, Detection of ciprofloxacin in urine through sensitized lanthanide luminescence, Sensors, 2016, 16, 2065.(Impact Factor = 3.9)
S. Singha, D. Kim, B. Roy, S. Sambasivan, H. Moon, A. S. Rao, J. Y. Kim, T. Joo, J. W. Park, Y. M. Rhee, T. Wang, K. H. Kim, Y. H. Shin, J. Jung, K. H. Ahn, A structural remedy toward bright dipolar fluorophores in aqueous media, Chemical Science, 2015, 6, 4335-4342.(Impact Factor = 8.4)
S. Singha, D. Kim, H. Moon, T. Wang, K. H. Kim, Y. H. Shin, J. Jung, E. Seo, S.-J. Lee, K. H. Ahn, Toward a selective, sensitive, fast-responsive, and biocompatible two-photon probe for hydrogen sulfide in live cells, Analytical Chemistry, 2015, 87, 1188-1195.(Impact Factor = 7.4)
D. Kim†, H. Moon†, S. H. Baik†, S. Singha† (†co-first author), Y. W. Jun, T. Wang, K. H. Kim, B. S. Park, J. Jung, I. Mook-Jung, K. H. Ahn, Two-photon absorbing dyes with minimal autofluorescence in tissue imaging: Application to in vivo imaging of amyloid-beta plaques with a negligible background signal, Journal of the American Chemical Society, 2015, 137, 6781-6789.(Impact Factor = 15.0)
S. Singha, D. Kim, H. Seo, S. W. Cho, K. H. Ahn, Fluorescence sensing systems for gold and silver species, Chemical Society Reviews, 2015, 44, 4367-4399.(Impact Factor = 46.2)
M. Tasior, D. Kim, S. Singha, M. Krzeszewski, K. H. Ahn, D. T. Gryko, π-Expanded coumarins: Synthesis, optical properties and applications, Journal of Materials Chemistry C, 2015, 3, 1421-1446.(Impact Factor = 6.4)
S. Singha, D. Kim, S. Rao, T. Wang, K. H. Kim, K. Lee, K. Kim, K. H. Ahn, Two-photon probes based on arylsulfonylazide: Fluorescence detection and imaging of biothiols, Dyes and Pigments, 2013, 99, 308-315.(Impact Factor = 4.5)
A. S. Rao, S. Singha, W. Choi, K. H. Ahn, Studies on acedan-based mononuclear zinc complexes toward selective fluorescent probes for pyrophosphate, Organic & Biomolecular Chemistry, 2012, 10, 8410-8417. (Impact Factor = 3.2)
D. Kim, S. Singha, T. Wang, E. Seo, J. H. Lee, S. J. Lee, K. H. Kim, K. H. Ahn, In-vivo two-photon fluorescence imaging of fluoride with a desilylation-based reactive probe, Chemical Communications, 2012, 48, 10243-10245.(Impact Factor = 4.9)

@ IIT Kharagpur

L. Rajput, S. Singha, K. Biradha, Comparative structural studies on homologues of amides and reverse amides: Unprecedented 4-fold interpenetrated quartz network, new beta-sheet, and two-dimensional layers, Crystal Growth & Design, 2007, 7, 2788-2795.(Impact Factor = 3.8)

Conference Paper:

U. Samanta, S. Ghosh, S. Singha*, Development of pH-Sensitive Colorimetric Polymer Film using Chitosan and Congo Red, in the Proceedings of the International Conference on “Exploring Molecules, Materials and Bio-materials for Sustainable Society (EMMBSS)”, Organized by Midnapore College, West Bengal & sponsored by SERB, India, 08-10 Sep, 2022, pp.31 (ISBN: 978-81-954721-3-0).
U. Samanta, S. Singha*, Quantifications of Endogenous Formaldehyde Levels in Mouse Organ Tissues through Two-Photon Fluorescence Bioimaging, in Proceedings of the 1st Virtual International Conference on “Biotechnology and Bioinformatics (ICBB-2022)”, Organized by Insight BioSolutions, France in technical collaboration with Indian Science and Technology Foundation (ISTF), New Delhi, 26-27 Feb, 2022, pp.22.
S. Singha, Y. W. Jun, J. Bae, Y. J. Reo, K. H. Ahn, Ratiometric tissue imaging for formaldehyde detection inmouse intestinal crypts, in Proceedings of the 3rd International Conference on Sensors Engineering and
Electronics Instrumentation Advances (SEIA’ 2017), 20-22 September, 2017, Moscow, Russia, pp.220-
222.

Book Chapter:

S. Singha*, ‘Fluorescence detection of ciprofloxacin’ in M. S. Attia (ed.),Ciprofloxacin: Biosynthesis,Applications, and Adverse Effects, Hauppage, NY: Nova Science Publishers,2019, pp.265-293.

Patents:

D. Chakraborty, B. Mandal, S. Singha, Anemometer-Based Fluid Flow Measuring System, Indian Patent Publication, Application No.: 202231076412 A, (Publication Date: Jan 06, 2023)
K. H. Ahn, H. G. Ryu, Y. W. Jun, K. H. Kim, S. Singha, Amino-Si-pyronin compound-based one- or two-photon absorption fluorescent substance and use thereof, United States Patent Application Publication, Patent Application No: US 16/642,439 (Publication Date: Aug 6, 2020).
K. H. Ahn, Y. W. Jun, H. Kim, S. Singha, Y. J. Reo, Benzocoumarin based two-photon absorbingfluorescent dyes, United States Patent Application Publication, Patent Application No: US15/955,877 (Application date: 18.04.2018).
K. H. Ahn, H. G. Ryu, Y. W. Jun, K. H. Kim, S. Singha, One- or two-photon absorbing fluorescent dyes
based on amino-Si-pyronin compound and the uses thereof; Organization: Korean Intellectual Property
Office, Patent Application No: KOR 10-2018-0102776 (Application date: 30.08.2018).
K. H. Ahn, D. Kim, H. Moon, S. Singha, B. Roy, S. Sunderraman, Novel two-photon absorbedfluorophore and cellular imaging method using the same, United States Patent Application Publication, Patent Application No: US 15/512,624 (Application date: 20.03.2017).
K. H. Ahn, Y. W. Jun, H. Kim, S. Singha, Y. J. Reo, Benzocoumarin based two-photon absorbing
fluorescent dyes, Organization: Korean Intellectual Property Office, Patent Application No: KOR 10-2017-
0078999 (Application date: 22.06.2017).
K. H. Ahn, D. Kim, S. Singha, One-photon and/or two-photon fluorescent probes for hydrogen sulfide,biological imaging method of hydrogen sulfide using the same and synthesis method of the same, United States Patent Application Publication, Patent Application No: US 15/037,168(Application date: 17.05.2016).
K. H. Ahn, D. Kim, H. Moon, S. Singha, B. Roy, S. Sunderraman, Novel two-photon absorbed
fluorophore and cellular imaging method using the same, Awarding Organization: Korean Intellectual
Property Office, Registration number: KOR 10-1662444 (Registration date: 27.09.2016).
K. H. Ahn, Y. W. Jun, J. Bae, H. G. Ryu, S. Singha, Probes for detecting formaldehyde or measuring
formaldehyde concentration, two-photon ratiometric fluorescence imaging and concentration
measurement of formaldehyde in cell or tissue using the same, Organization: Korean Intellectual
Property Office, Patent Application No: KOR 10-2016-0124841 (Application date: 28.09.2016).
K. H. Ahn, D. Kim, S. Singha, One-photon and/or two-photon fluorescent probes for hydrogen sulfide,
biological imaging method of hydrogen sulfide using the same and synthesis method of the same,
Awarding Organization: Korean Intellectual Property Office, Registration number: KOR 10-1481921
(Registration date: 06.01.2015). # Technology transferred & license agreement for commercialization
with Merck Inc., USA.

Administrative Experience:

Prof-in-charge- IPR & Innovation Cell, JIS Institute of Advanced Studies and Research (JISIASR) Kolkata (2022 – Present)
Prof-in-charge- Industry-Institute Collaboration, JIS Institute of Advanced Studies and Research (JISIASR) Kolkata (2019 – Present)
Member of JIS Research Council (2020 – Present)
Faculty in-charge of Departmental Faculty Council/Affairs, Centre for Interdisciplinary Sciences, JIS Institute of Advanced Studies and Research (JISIASR) Kolkata (2020 – Present)
Faculty in-charge of Lab Development for PG Program/Research (Departmental), Centre for Interdisciplinary Sciences, JIS Institute of Advanced Studies and Research (JISIASR) Kolkata (2021 – Present)

Professional Responsibilities:

Organizing Secretary of Indo-Korean Joint Two-Days Online International Workshop on Advanced Functional Materials (July 15-16, 2021) organized by JIS Institute of Advanced Studies and Research (JISIASR) Kolkata, JIS University, India & Hongik University, Republic of Korea.
Coordinator of the National Science Day 2020 program and event organized by JIS Institute of Advanced Studies and Research (JISIASR) Kolkata, JIS University, India (28th Feb, 2020).

Membership of Professional Bodies:

Community Member (Membership Number: 31340778) of the American Chemical Society (ACS), USA (2020-present).
Affiliate Member (Membership ID: 704682) of the Royal Society of Chemistry (RSC), London, UK (2021-present).

Honors, Awards, & Achievements:
@ JISIASR, JIS University, Kolkata

Recognized as Outstanding Reviewer for Journal of Materials Chemistry B 2022 by Royal Society of Chemistry (RSC), London.
Recognized as Outstanding Reviewer for New Journal of Chemistry 2022 by Royal Society of Chemistry (RSC), London.
Awarded research grant as Principal Investigator under Core Research Grant (CRG) of SERB, DST, India, Project title: Development of two-photon fluorescent probes for proto-oncogenic kinases with potential applications in anticancer drug screening and targeted drug delivery (Nov, 2022)
Recognized as Outstanding Reviewer for New Journal of Chemistry 2021 by Royal Society of Chemistry (RSC), London.

@ POSTECH, South Korea

Awarded research grant as Principal Investigator under Individual Research in Basic Science and Engineering supported by National Research Foundation (NRF), South Korea, Project title: Hydrogen sulfide triggered drug delivery for theranostics, Award No.: 2018R1D1A1B07051403 (Jun, 2018-May, 2023)
Post-Doctoral Research fellowship from Global Research Laboratory (GRL) program, South Korea (Mar, 2015-Sep, 2015)
Post-Doctoral Research fellowship from Center for Electro-Photo Behaviors in Advanced Molecular Systems (EPB Center), South Korea (Aug, 2013-Feb, 2015)

@ IIT Kharagpur, India

MCM (Merit-cum-Means) Scholarship from IIT Kharagpur (2005-2007)
CSIR-UGC NET (National Eligibility Test) Examination qualified (Date: 24th Dec, 2006) in India
All India Rank 59th in JAM (Joint Admission Test for M.Sc. in IITs), 2005

Editorial Experiences:

Associate Editor of Frontiers in Environmental Chemistry (2022-present).
Review Editor of Frontiers in Environmental Chemistry) (2020-2022).
Topic Editor of “Liquids” and “Polymers” (MDPI publications) (2020-Present)
Lead Guest Editor of Journal of Analytical Methods in Chemistry (Hindawi Publishing Corporation) for the special publication issue on “Fluorescence analysis: From sensing to imaging” (2017-2018)
Academic Editor of Journal of Analytical Methods in Chemistry (2017-2018)
Reviewed over 200 manuscripts (verified by Web of Science) for the various journals from the renowned publishers such as – American Chemical Society (ACS), Royal Society of Chemistry (RSC), Elsevier, Wiley, Springer Nature, MDPI (Switzerland), etc.

DR. SUBHANKAR SINGHA